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Characterization of nanoparticles and microneedles The surface morphology of ZC-MSN and was observed using scanning electron microscope (SEM, S-4800, Hitachi, Japan) and transmission electron microscope (TEM, JEM-2100F, JEOL, Japan), while the element content (O, Si, Cu, Zn) in ZC Nanoparticles was detected by SEM accessory energy-dispersive spectrometer (EDS) system

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