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Beyond its popular cosmetic benefits, it acts as a signaling molecule that modulates gene expression, potentially influencing collagen production and tissue repair

After quantifying the concentrations of melatonin, serotonin, dopamine, glutamate, and neurotrophic factors using HPLC and ELISA, the results are analyzed using statistical software such as GraphPad Prism

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A Youdens J statistic capillary cutoff (0.016 pg ml 1 ) led to a PPV of 0.849 (95% CI 0.7580.909) and an NPV of 0.711 (95% CI 0.6100.795), at a sensitivity of 73.7% and specificity of 83.2%