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Chromatographic separation was conducted on a Brownlee SPP C18 column (2.1 mm 75 mm, 2.7 m particle size, Perkin Elmer, Waltham, MA) using mobile phase A (100% H2O containing 0.1% formic acid and 1% of 1 M NH 4 OAc) and B (1:1 acetonitrile:isopropanol containing 0.1% formic acid and 1% of 1 M NH 4 OAc)

Matrix metalloproteinase and proinflammatory cytokine production by chondrocytes of human osteoarthritic cartilage: associations with degenerative changes

Addressing these mechanisms requires advanced methodologies, including single-cell multi-omics and spatial transcriptomics, to dissect the interplay between innate and adaptive compartments at lesion sites