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Dietary supplementation with a novel l-carnitine multi-micronutrient in idiopathic male subfertility involving oligo-, astheno-, teratozoospermia: A randomized clinical study

Funding Red de Salud Materno-Infantil y del Desarrollo (SAMID) and Instituto de Salud Carlos III (ISCIII) (RD12/0026/0003 and RD16/0022/0002), co-funded by the European Union (PI16/00566 and PI19/01853) and Redes de Investigacin Cooperativo Orientadas a Resultados en Salud (RICORS) (RD21/0012/0017)

The experimental animal, properly identified by its weight number, should be securely restrained by an assistant, positioning the animal with its head downward and abdomen upward

48 h after transfection, cells were washed twice with pre-warmed PBS and fixed with 4% paraformaldehyde (PFA) for 1015 min