Janky R, Varga V, Hermann A, Saransaari P, Oja SS
It has a long history of use as a botanical remedy dating back to the fourth century BCE, when ancient Greeks regarded it as a general medicinal herb.1 Since then, it has been used to treat an array of ailments, including but not limited to: Hepatitis Cirrhosis Jaundice Diabetes The active ingredient in milk thistle is silymarin, a group of flavonoids that comprises silybin, silychristin, isosilybin, and silidianin.11 Silymarin is whats known as a free radical scavenger, otherwise known as an antioxidant
Cells were then stained with 0.1 g ml 1 Hoechst 33342 (Invitrogen, H3570) for 20 min at 37 C followed by treatments as described in individual experiments with 5 nM SYTOX Green to monitor cell death
caution and monitoring are advised