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2.3 Global Untargeted Metabolomics Analysis 2.3.1 Preparation of NIST Serum Standard Curve Correction Solutions Initially, 5, 10, 50, 100, 200, and 300 L NIST serum (National Institute of Standards and Technology, Standard Reference Material 1950) was added to centrifuge tubes, respectively, followed by the supplement of ddH2O, mixed internal standard solution, and methanol solution (ThermoFisher Scientific, USA) to a certain proportion

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