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The use of these proteins would be based on their expected biological activity: DSIP as the active peptide expected to induce sleep, and CBBBP to facilitate entry into brain

Optical density of the obtained supernatant was read at 532 nm and level of mtLPO was expressed as nmol MDA/mg protein (molar extinction coefficient 1.56 105 M 1 cm 1 )

Based on these results, we propose that inhibition of RNR (or upstream enzymes) leads to accumulation of GSH which can be re-purposed for use by GPX4 to prolong the inhibition of ferroptosis under conditions where new GSH synthesis is otherwise impossible (Fig 6)