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On the fourth day, 1 mg ml of l-(4, 5-Dimethylthiazol-2-yl) 3, 5-diphenyIformazan reagent (MTT, Sigma- Aldrich) prepared in PBS was added to the cells and incubated for 4 h

In essence, it functions as a signaling molecule: when introduced into damaged tissue, it appears to activate multiple healing pathways that help the body repair itself more efficiently

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