Botanical Garden Blot (Green Algae, Yeast, Barley, Sweet Corn, Rye Grass, Oat, Wheat, Soy, Mung, Sunflower, Cotton, Beet, Tomato, Spinach) size:1 Blot Five µL of each reaction

Five µL of each reaction were resolved by agarose gel electrophoresis and visualized by staining with ethidium bromide

30% deionized water

Intramolecular ligation of single-stranded DNA ends containing 5'-phosphates and 3'-hydroxyl groups

less lysozyme is needed in a reaction

Botanical Garden Blot (Green Algae, Yeast, Barley, Sweet Corn, Rye Grass, Oat, Wheat, Soy, Mung, Sunflower, Cotton, Beet, Tomato, Spinach) size:1 Blot Five µL of each reactionThe blot is prepared using total protein extracted from a variety of 5 10 day old, whole plant tissues. The premade blots are prepared with the appropriate GenLysate and 50g protein is loaded onto a 4 20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer Buffer. The blots are ready to be blocked and probed with the