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in some cases expression is minimal or not detectable because the recombinant protein
Ligation amplification (Ligase Chain Reaction
with Bacillus genomic DNA (+) or no DNA (-) as a template (1450 bp product expected)
Poly(A) tailing of RNA with the Poly(A) Polymerase Tailing Kit
BAC-Optimized Replicator-v2.0 Electrocompetent Cells (DUOs) size:24 rxns in some cases expression isBAC Optimized Replicator v2. 0 & 10G BAC Optimized Electrocompetent Cells Cells that enable the most challenging library construction and screening. Highest available transformation efficiencies for library construction and screening. Optimized for high yields of DNA. Lucigen offers two bacterial strains designed for BAC library construction or transformation of large constructs: BAC Optimized Replicator v2. 0 Electrocompetent Cells (> 1 1010 cfu g
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