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25(OH)VD, 1,25(OH) 2 VD, VDBP, GSH, TNF-, PTH, insulin, glucose, protein carbonyl, and MDA assays Plasma levels of 25(OH) vitamin D were determined using an ELISA kit (Calbiotech, Spring Valley, CA) and 1,25(OH) 2 vitamin D using another ELISA kit (My BioSource, San Diego, CA)

It is emerging [6] that the primary upstream factor which links endothelial dysfunction with CVD and T2DM and described as cardiometabolic disease is closely related to oxidative stress [611]

The mice were allowed free access to food and water and were maintained on a 12-h lightdark cycle at room temperature (24 2 C) with constant humidity (40% 15%)

Unreconstituted powder can be stored refrigerated or frozen for extended periods