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Double or triple primary antibody applications were performed in Block-B buffer overnight at 4 C with dilutions as follows: KRT5 (1:500), TP63 (1:200), MKI67 (1:100), KRT8 (1:100), MUC5AC (1:500), MUC5B (1:200), KRT14 (1:200), DMBT1 (1:50), SCGB3A1 (1:20), ACTA2 (1:100), SCGB1A1 (1:500)

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Dozens of artists have covered his work, including Elvis Presley, Barbra Streisand, Harry Belafonte, Johnny Cash, Anne Murray, Janes Addiction, Sarah McLachlan and, perhaps most surprisingly, dance supergroup Stars on 54 who turned his classic If You Could Read My Mind into a disco-pop curiosity for the 1998 movie 54. Most of his songs are deeply autobiographical with lyrics that probe his own experiences in a frank and unclouded manner and explore issues surrounding the national identity

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