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To reduce irritation and minimize the risk of scar tissue, its best to rotate between injection sites rather than using the same spot every time

Similarly, we used the CY5 Fluorescent Light Cube and DAPI Fluorescent Light Cube to capture triplicate images of each well and analyzed in the open-source ImageJ platform Fiji [109]

In the following section, we translate these mechanistic findings into clinical applications, evaluating emerging strategiesincluding ferroptosis inhibitors, iron chelation therapies, and biomarker-driven approachesdesigned to disrupt this pathological cycle and modify the progression of COPD