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Mitochondrial membrane potential (MMP) The MMP was measured in ES2 and OVCAR3 cell lines using JC-1 (M34152, Molecular Probes, Life Technologies) 58 , a specific mitochondrial probe, which becomes red when it enters polarized mitochondria and it is green in cytoplasm when mitochondrial potential is lost avoiding its entrance

Levels of specific transcripts were given by the threshold cycle (Ct) values and the data were normalized for the corresponding -actin RNA contents

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It consists of three amino acidsglycine, cysteine, and glutamic acid and offers a range of health benefits such as liver detoxification, improved immune system function, and powerful antioxidant defense