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More immigrants arrived after the English responded forcefully to the 1644 uprising and destroyed most Native American towns near the settled area, increasing the sense of personal security in the colony at the same time the Civil War in England reduced personal security at home

Thus, the Spaniards brought more and more enslaved people in to replace the native workers, and each ship carried the risk of another epidemic

After ending the flue-curing process, the relative contents of Ascomycota, Basidiomycota and the other fungal phyla were 83%, 5% and 12%, respectively, which were basically similar to the results under conventional procedure