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For lyophilized vials, recommended freezer storage at 20 C or lower is cited

Frequently Asked Questions About Vitamin B12 & Nutrient Injections What are the benefits of B12 injections compared to oral supplements

Excessive secretion of corticotropin-releasing hormones (CRH) in patients with depression increases CRH in the blood, cerebrospinal fluid, and metabolites, which downregulate the CRH receptor and cause the loss of the action site of excessive secretion of CRH, resulting in a vicious cycle of increasing CRH in the body and eventually producing depressive symptoms [107]

Lockhart JN, Stevens DM, Beezer DB, Kravitz A, Harth E