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Shaking introduces air bubbles and creates cavitation forces (micro-scale pressure waves) that denature peptide chains

[1] Spike-timing-dependent plasticity (STDP), where a presynaptic neuron firing just milliseconds before a postsynaptic neuron leads to synaptic strengthening (timing-based long-term potentiation LTP), while firing after the postsynaptic neuron causes weakening (timing-based long-term depression LTD)

Magnesium glycinate is often recommended for better sleep, as the amino acid glycine also has a calming effect on the brain

[1] In its biologically active form the peptide is complexed with copper(II) to form GHK-Cu, a small, diffusible copper-carrier molecule