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The recommendation from the panel, however, is not final

After tandem purification using GST and strep affinity tags, we obtained the protein sample for cryo-EM data collection (Fig

Bibcode:2011Sci...334..941K

First, AX-protein conjugates were analysed by SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis) and Western blot using an anti-AX monoclonal antibody and, second, the content of low molecular weight compounds was studied by spectroscopic techniques and liquid chromatography