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Cells were then blocked with 3% BSA at room temperature for 1 h followed by incubation with a primary antibody E-cadherin, ZO-1, Keap1 and Nrf2 (1:100 dilution) at 4 overnight

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Canada reduced the illicit share of its market from nearly 30 percent in the early 1990s to between 7.6 percent and 14.7 percent in 2010 (by the committees estimate) through sweeping intervention efforts, including licensing, tax stamps, enforcement efforts, tax harmonization, tribal tax revenue agreements, legal agreements with tobacco manufacturers, and public education campaigns

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