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Right-sided colon cancers are frequently characterized by high microsatellite instability (MSI-H) and pronounced immune infiltration, accompanied by a glycolysis-dominant metabolic profile

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To determine whether this shift in molecular weight reflects glycosylation associated with PLA2G2F secretion, we treated both cell lysates and conditioned medium samples with PNGase F to remove N-linked glycans

Supplement conditions consisted of isoenergetic placebo and glutamine (0.3 gkg-1day-1 + 0.3 gkg-1day-1 maltodextrin) ingestion once per day over 72 hr