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J Biol Chem 272 : 1664416651 Kingsley PD, Whitin JC, Cohen HJ, Palis J 1998 Developmental expression of extracellular glutathione peroxidase suggests antioxidant roles in deciduum, visceral yolk sac, and skin

15 g of lysates were reduced in NuPAGE reducing reagent (Invitrogen) and boiled for 5 min prior to loading in 4-12% NuPAGE Bis-Tris Gel for separation by electrophoresis