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It offers a simple way to manage B12 needs and often works well to address deficiencies caused by diet

1- Free GSH=DTNB+Sample+Substrate 2- Total GSH=DTNB+Sample+Substrate+ NADPH+glutathione reductase 3- GSSG=Total GSH-Free GSH Another method to measure GSSG separately, is to remove the free GSH from DTNB reaction process by modification of free GSH with N-ethylmaleimide (NEM) or vinylpyridine prior to conducting assay

But depending on your symptoms and what type of anemia your healthcare provider thinks you may have, you may need other tests

Effects on specific populations including the elderly, those with metabolic disease, and women have not been specifically investigated