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Tetrahydrobiopterin can be regenerated in a salvage pathway that involves the enzymes pterin 4-carbinolamine dehydratase 1 and dihydropteridine reductase

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At a determined period of time (e.g., 6 hours, 12 hours, 1 day, 2 days, 3 days, 4 days, 5 days, 6 days, 7 days, and 14 days for testing the pharmacokinetics of a formulation or composition over a 1 or 2 week period), the test animal is euthanized and the inner ear removed and tested for the presence of the otic agent