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To further explore the underlying mechanism of GSH in activating systemic plant defense responses, we introduced the [Ca 2+ ] cyt reporter GCaMP3 into pad2-1 , generating GCaMP3 / pad2-1 plant, to investigate whether the GSH-deficient mutant attenuated the wound-induced systemic [Ca 2+ ] cyt transmission

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Finally, the mold was transferred to an oven (45 C) and placed for 72 h to for demolding, The pure microneedles (MN), Qu loaded microneedles (Qu/MN) and ZC-MSN loaded microneedles (ZC/MN) were also prepared as control groups follow the same protocol using the corresponding ingredients with the same amounts

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