Oxidative stress has been proposed to play a critical role in the pathogenesis of tinnitus, since it could lead to cellular changes in hair cells, hair cell apoptosis, cochlear degeneration, changes in supporting structures and stria vascularis, changes in nerve fibers of the acoustic nerve, irregular neural activity in the auditory pathway and dysfunction of the central cortex [3,4,5]
In nucleated cells, their functions are not limited to apoptosis, and caspases have been demonstrated to be involved in the regulation of multiple RCDs and related mechanisms, including pyroptosis (caspase-1), necroptosis (caspase-8), mitotic catastrophe (caspase-2), and regulation of inflammation [4]
Research is now searching for molecules with the same recognition sequences and structures to allow the same cell reactions
Cells were then changed to fresh growth medium until harvest