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First, RHEB was incubated with liposomes at concentrations of 0.25 M and 0.18 mM, respectively, at 4 C overnight in 40 l lipid buffer

Among the remaining subjects, we further excluded those who received a diagnosis of bipolar disorder during the follow-up assessment (n = 9), those with missing metabolite data due to the lack of blood samples (n = 158) and those who did not complete the Inventory for Depressive Symptoms questionnaire (n = 33)

Holmuhamedov E, Lewis L, Bienengraeber M, Holmuhamedova M, Jahangir A, Terzic A

The main benefit is moving from a single-target treatment to a comprehensive support system