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These enzymes are fast and versatile, able to use a wide range of substrates, but the requirement for H 2 O 2 means that the labeling conditions are toxic, especially in tissue and animals

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Cellular GSH is found mostly in the cytosol, with the remainder spread across organelles ( The thioredoxin system is composed of Trx proteins and Trx reductases (TrxR), in which the reduced form of Trx proteins (Trx(SH) 2 ) are disulfide reductases as they contain dithiol groups in their highly conserved active site (-Cys-Gly-Pro-Cys-) ( 2 can directly reduce oxidized proteins or provide electrons to thiol-dependent peroxidases (Prx) to convert hydrogen peroxide to water