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Blind cage coding and automated video analysis (EthoVision, TopScan) are recommended Too low n : prior power analysis, n 8 minimum for rodent behavioural tests No positive control : without memantine, donepezil or scopolamine depending on the paradigm, impossible to judge if the model is functional No dose-response : a single tested dose is insufficient to conclude

Another outstanding question Bluestone introduces is whether some of the patients experiencing specific irAEs have some aspects of the disease prior to immunotherapy (pre-existing condition) such that the treatment exacerbates it

Moreover, pre-treatment with anti-MET antibodies blocked the beneficial effect exerted by HGF, while the one exerted by MSCs was blocked by anti-HGF antibody, showing that the positive effect was mostly dependent on the production of HGF

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