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Briefly, individual mice were administered 15 g each of RNAi agent with In vivo-jet PEI (Polypulus Transfection Inc, New York, NY) (resulting in a calculated 1:6 charge ratio of nucleic acid backbone phosphates to cationic lipid nitrogen atoms) in a volume of 25 l on day 1 using an endotracheally intserted MicroSprayer aerosoliser (IA-1C

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doi: 10.1136/thoraxjnl-2015208039 11 ChenEYSunAChenCSMintzAJChinWC

Furthermore, previous owners were interviewed about smoking behaviors, including variables such as number of cigarettes smoked per day both inside and outside the car as well as use of ventilation, such as rolling down the windows or turning on the air conditioner