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Other research on the effects of these cigarettes in high-risk groups was limited by very small samples (for example, only six individuals with affective disorders) and no assessment of chronic exposure to only these cigarettes

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National Toxicology Program released a Strategic Roadmap advising regulatory agencies to provide more human-relevant toxicology data while reducing the use of animals. Currently, the CDC has numerous options for studying vaping without animals, including in vitro assays, in silico approaches, computational chemistry and a range of sophisticated tissue models that include 3D organoids and organs-on-chips