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For biochemical assays, the sciatic nerve samples were washed with saline, homogenized on ice, and centrifuged at 12,000 rpm for 20 minutes at 4 C

At this time, the PHE is still in place

Therefore, two improvements are mandatory for improved chemotherapy 1) novel delivery techniques that can target cancer cells to deliver anticancer drugs and 2) methods to specifically enhance drug efficacy within tumors

The FXR agonist fexaramine not only restores intestinal FXR activity in mice induced by deoxycholic acid(DCA), but also regulates the abundance of short-chain fatty acid-producing bacteria, thereby alleviating intestinal inflammation [476]