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However, the underlying mechanism by which MTX provokes tissue injury is not yet well known, and direct toxic effects of MTX are thought to be caused by excessive generation of free radicals, causing an imbalance between free radical production and antioxidant defense, which finally results in the development of oxidative stress (Drishya et al

The mitochondrial permeability transition porecurrent knowledge of its structure, function, and regulation, and optimized methods for evaluating its functional state

It is clinically known for reducing visceral (deep abdominal) fat and improving metabolic health

doi:10.1016/0960-0760(93)90022-o