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For lysosomal enzyme assays, 600 g of whole cell lysates prepared from either WT HEK293T cells, or from untransfected or 48 h transfected LYSET knockout, GNPT knockout, or double knockout cells were incubated with equivalent amounts of the CI-MPR affinity beads for 2 h at 4 C, washed twice with cold Tris-buffered saline containing 1% Tx-100 (pH 7.4) (TBST), then assayed for bound lysosomal enzymes

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The 0.9% concentration is calibrated to provide effective bacteriostasis at the anticipated microbial challenge levels associated with multi-use vial access in laboratory research environments while remaining below the concentration ranges associated with peptide and protein stability perturbation in reconstituted research compound solutions

All treatments are performed under strict clinical standards by qualified nurses and doctors