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Shaking can cause mechanical stress that damages the delicate peptide chains, potentially denaturing them and rendering them ineffective

Footnotes References 1 AlamroA

Supplementation has been linked to increased expression of slit diaphragm proteins such as nephrin and podocin, as well as reduced tubular injury, possibly through anti-inflammatory and anti-RAAS mechanisms ( Taken together, these findings suggest that vitamin D exerts renal protection in CKD not only through inflammation, oxidative stress, and fibrosis regulation but also via its roles in autophagy, Klotho signaling, and podocyte stability, highlighting its broader therapeutic potential (Figure 3)

doi: 10.1016/j.biotechadv.2021.107794 33 Chvez-CabreraC.Flores-BustamanteZ