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The safest approach is using on alternate days

The levels of positive staining were quantified as the mean fluorescence intensity using ImageJ software [21]

In the contrary, an improvement in hyperpigmented areas in glutathione gel with noisome treated right side was reported in the form of decreased shades of pigmentary network in cases of epidermal melasma and decreased irregularity of pigmentary network, decreased number of granules in mixed melasma, this agrees with Javed and colleagues , who used dermoscopy and Woods light in their studies

We statistically compared the similar variances between the groups as well