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The functionality of this prototype was evaluated by 1) visual evaluation of the generated plasma spots ensuring that the 3D card is capable of producing hemolysis-free plasma spots and 2) automated on-line analysis using the previously validated DPS-SPE-LC-ESI-MS/MS (dried plasma spot-solid phase extractionliquid chromatography-electrospray ionizationtandem Mass Spectrometry) method for quantitative determination of hydrocodone using deuterium-labeled internal standard

Dihexa is not FDA-approved for any indication and has no published human safety data

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