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At the completion of the incubation, the cells were washed three times with ice-cold phosphate-buffered saline (PBS), and then, the fluorescence was measured by fluorescence microscopy at an emission wavelength of 460 nm using an excitation wavelength of 360 nm for the DCF fluorophore

Mixing different peptides in the same vial during reconstitution is not recommended

However, pregnancy brings changes to nutrient balance and metabolism, and supplementation may affect that balance

The neuroprotective effects may be related to DSIP's sleep-promoting properties, as adequate slow-wave sleep is essential for brain waste clearance through the glymphatic system and neural repair processes