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The cells were harvested and the cell pellet was resuspended in lysis buffer (300 mM NaCl, 50 mM Tris-HCl pH 7.5) followed with sonication and centrifuged at 13,000 rpm to clarify the supernatant

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Resveratrol was shown to decrease IL-1 and tumor necrosis factor-[105] expression and reduce iNOS expression, COX-2 messenger RNA, and production of IL-8 and GM-CSF[106] in human lung epithelial cells More studies need be done to understand how resveratrol fits into inflammatory pathways in the development of novel therapies for the treatment of asthma