It is not approved for human or veterinary use, clinical administration, therapeutic intervention, or diagnostic procedures
On non-training days, consistency matters more than specific timing maintaining the same daily injection time (morning or evening) preserves stable plasma concentrations and simplifies protocol adherence across multi-week cycles
Justice et al., 1 is prominently expressed in the forebrain including the isocortex throughout cortical layers II-VI, hippocampal formation at the CA1 level, basal ganglia within the globus pallidus and striatum, sensory systems and the amygdala (Justice et al., 1 was also detected in all segments of the mouse spinal cord throughout laminae II-V (Korosi et al., 2 shows a wide distribution in the brain, most notably in the amygdala, lateral septum, supraoptic nucleus, ventromedial hypothalamus, dorsal raphe nuclei, area postrema, the nucleus of the solitary tract and the spinal cord (Bittencourt and Sawchenko, Brain somatostatin signaling Somatostatin-14 was isolated in 1973 from ovine hypothalami and shown to inhibit growth hormone secretion in vitro (Brazeau et al., Besides the initially described expression site in the hypothalamus, somatostatin is widely distributed in the rodent brain with dense expression in the cortex, amygdala, limbic and sensory system, periaqueductal central gray and paraventricular, ventromedial and arcuate hypothalamic nuclei (Finley et al., 2008)
These observations show the statistical limitations of small sample sizes (n=3-4/group) but support the consistency in the trends observed
