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Five sets of experiments on cell cultures were performed: with 5 mM glucose (NG), NG + 100 nM celastrol, 30 mM D-glucose (HG), HG + 100 nM celastrol, and HG + 1 M Fer-1

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By upregulating and activating SIRT1, which subsequently deacetylates and functionally inhibits STAT3, GHK-Cu entirely short-circuits the inflammatory cascade, promotes profound mucosal healing, and restores the integrity of tight junction proteins (such as ZO-1 and Occludin) within the cellular barrier

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