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For the monolayer culture (1d-PHH monolayer control), the cells were cultured on Williams E medium supplemented with 1% HEPES + 1% GlutaMAX + 1% penicillin/streptomycin and 100 nM dexamethasone for 18 h (or 24 h, for albumin assays) and then processed for the functional assays

130 SIRT1 deacetylates NF-B, inhibiting pro-inflammatory cytokines (IL-6, TNF-) while promoting anti-inflammatory cytokines (IL-10), 35 limiting Th17 and Tc activation and supporting overall immune balance

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Unlike minoxidil, which can cause unwanted facial hair through systemic absorption, topical copper peptides applied to the scalp typically don't cause facial hair growth