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Overall, results indicate that physical disruption of the disc matrix can lead to focal swelling, GAG loss, disturbed cell-matrix binding, cell clustering, and a degenerative cell phenotype

It modulates nitric oxide pathways to normalize lower esophageal sphincter function, reduces oxidative stress markers like malondialdehyde, and decreases inflammatory cytokine expression (TNF-, IL-6)

The protocol from Celsis for assessing microsomal-dependent drug metabolism was followed with minor adaptations