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To prepare the Transwell assay, murine melanoma cells (1 10 5 cells per insert) cultured in 200 l RPMI1640 medium supplemented with 0.5% FBS were plated on the upper insert (6.5 mm diameter transwell with 8 m pores, Corning), while 800 l RPMI1640 medium supplemented with 0.5% FBS, with or without hHGF or dimeric macrocycle was added to the bottom chamber

Liquid-liquid extraction, either with acetonitrile based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) protocols or by salting-out assisted extraction with ethyl acetate are other commonly used sample preparation techniques used also for large cohorts (Njumbe Ediage et al

Reduced function of GPX4 is frequently observed in degenerative disorders

10.1074/jbc.274.17.11455 118 SavaskanN