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Exogenous proline application ameliorates toxic effects of arsenate in Solanum melongena L

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To investigate the effect of gene knockout on l-cysteine and GSH degradation, the engineered strains were cultured in the flasks as described above and the cells were collected by centrifugation (6000 rpm, 4 C for 5 min) after two washes with 50 mM phosphate buffer (pH7.0)

AI techniques such as Monte Carlo Tree Search, Hidden Markov Models, and Variational Autoencoders guide sequence generation, followed by secondary structure prediction and molecular dynamics simulations for interaction validation