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equal volumes of whole blood and 6% (w/v) hetastarch in 0.9% NaCl is a third option [7]

In contrast to histamine which is preformed in a one step reaction by decarboxylation of histidine predominantly in mast cells and basophils, cysteinyl leukotrienes (CysLTs) represent a class of immune-modulating eicosanoids which are rapidly synthesized de novo in a multi-step cascade in response to various stimuli mainly in basophils, eosinophils and mast cells and to a lower extend in macrophages and dendritic cells [14, 15]

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While ferroptosis and gut dysbiosis have been extensively studied in isolation, growing evidence supports profound molecular crosstalk between these pathways, which synergistically drive OA progression