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Estradiol mediates fluctuation in hippocampal synapse density during the estrous cycle in the adult rat

Add the outer thighs and upper arms, and you have weeks of unique injection locations before needing to revisit any area

GSH concentration is instead measured by either of two methods: by analysis of GS-NEM conjugates by HPLC in the same sample that is used to measure GSSG or, alternatively, by analysis of GSH by spectrophotometry (GSH recycling method) on one additional sample aliquot that has not been derivatized with NEM