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For reducing SDS-PAGE, 2 g of protein was mixed with 3 L of 4 Bolt LDS Sample Buffer (Invitrogen), 1.2 L of 10 Bolt Sample Reducing Agent (Invitrogen), and Milli-Q water to a total volume of 12 L, and the samples were incubated for 10 min at 95 C

Mechanistically, 4-HBGs neuroprotective efficacy against glutamate excitotoxicity has been attributed to competitive inhibition of kainate receptor binding ( 3.6 Brain tissue distribution of GEB extract in normal and MCAO rats Cerebral distribution analysis revealed distinct patterns of six GEB-derived compounds (PA, PB, PC, PE, GAS, 4-HBG) between normal and MCAO rats following oral administration (Figure 8)

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Stress can lead to an imbalance between light energy absorption and utilization, resulting in oxidative stress and consequently an increase in the content of reactive oxygen species (ROS) [e.g