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For metronidazole, Metro-P3 , MF-01 , MF-02 and MF-03 , the same extraction procedure was used: 7.5 l of a plasma sample (calibration samples, QCs or PK samples) were extracted with 37.5 l of a 50:50 mixture of acetonitrile and methanol containing 12.5 ng ml 1 of caffeine as internal standard for 15 min at 2,000 rpm on an Eppendorf MixMate vortex mixer