Western blotting Cell pellets were lysed in RIPA buffer (25 mM Tris-HCl, pH = 7.5, 150 mM NaCl, 0.1% Nonidet P-40, 0.5% sodium deoxycholate and 0.1% SDS) supplemented with protease inhibitor cocktail (Sigma) and phosphatase Inhibitor Cocktail 2 and 3 (Sigma)
The study reveals that BPC-157-induced GHR expression increases sensitivity to growth hormone signaling: adding growth hormone to BPC-157-treated fibroblasts dose- and time-dependently increased cell proliferation (MTT assay and PCNA) and activated the downstream Janus kinase 2 (JAK2) pathway
Applebury ML, Farhangfar F, Glsmann M, Hashimoto K, Kage K, Robbins JT, Shibusawa N, Wondisford FE, Zhang H
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