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Nelfinavir induces adipocyte insulin resistance through the induction of oxidative stress: differential protective effect of antioxidant agents

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For gene expression analysis, 1 g of total RNA for each sample was retrotranscribed into complementary DNA (cDNA) with the iScript TM cDNA Synthesis Kit (BIORAD, Hercules, CA, USA), following the manufacturer's instructions, using the GeneAmp PCR System 9700 (Perkin Elmer, Waltham, MA, USA)